Sweetener Introduction:

Sweetener is a general term for substances that can impart sweetness to food. Due to the high efficiency and cost-effectiveness of artificially synthesized sweeteners such as sodium saccharin (SA), saccharin (SC), acesulfame (AK), aspartame (ASP), neotame (NTM), and stevia glycosides (S), they are widely used in food. But excessive use can cause certain toxic side effects. For example, steviol glycosides may have certain carcinogenic effects; Aspartame can affect the development of patients with phenylketonuria; Long term consumption of saccharin sodium can lead to malnutrition, and its carcinogenic potential has not been completely ruled out; Sweetener may be carcinogenic, and its metabolite cyclohexylamine has toxic effects on the cardiovascular system and testes. In recent years, it has been discovered that some businesses are excessively using artificial sweeteners and some food labels do not indicate the use of artificial sweeteners. Some are confusing the public with terms such as "sugar syrup", "sweetener", "protein sugar", etc., in order to conceal the harm of their products and pose a threat to the health of consumers. This article aims to establish a simultaneous detection method for multiple sweeteners.

Sample processing procedure

A、 Simple sample medium:

For example, for carbonated beverages, fruit wine, grape wine, etc., weigh 10g of the sample (accurate to 0.001g), place it in a 25mL volumetric flask, dilute with water (if ethanol is present, heat it in a water bath to remove ethanol and then dilute with water to the original volume) to the mark, mix well, filter through a 0.45 μ m filter membrane, and test the filtrate on the machine.

B、 Samples with complex media

For example, for tea drinks, fruit juice, etc., weigh 6g of the sample (accurate to 0.001g), place it in a 25mL volumetric flask, dilute with water to the mark, centrifuge at 4000r/min for 10 minutes, take the supernatant, and test it.

C、 Sample with solid medium

For example, for candied fruits, pickled vegetables, etc., crush the sample. Weigh 2g of homogeneous sample (accurate to 0.001g), take an appropriate amount and place it in 25mL, add water and sonicate for 20 minutes, then add water to make up to 25mL. Centrifuge at 4000r/min for 10 minutes, take the supernatant and test it.

D、 Milk containing beverages and plant-based protein beverages

Weigh 6g of the sample (accurate to 0.001g) and place it in a 25mL volumetric flask. Add 0.08mol/L K4 [Fe (CN) 6] (pH4.4) and 0.25mol/L zinc acetate (pH4.5) solutions in sequence, shake vigorously for 2 minutes to precipitate the protein, add water to the mark, centrifuge at 4000r/min for 10 minutes, take the supernatant, centrifuge at 4000r/min for 10 minutes, take the supernatant for testing.

Purification of the sample

Extract 3mL of the supernatant from B, C, and D using a solid-phase extraction column (activated with 3mL of acetonitrile and equilibrated with 3mL of water). Then wash with ammonium sulfate aqueous solution and acetonitrile in sequence, collect the eluent in a volumetric flask, dilute to the mark with water, mix well, filter through a 0.45um filter membrane, and the filtrate is ready for measurement on the machine.

[Chromatographic conditions]

Chromatographic column: C18,

Detection wavelength: Detector: UV detector or diode array detector

Detection temperature: room temperature

Flow rate: 1mL/min

Injection volume: 20uL

Mobile phase: gradient elution

[Spectrum]

[Instrument Configuration]